INVESTIGADORES
LUCANGIOLI Silvia Edith
artículos
Título:
Simple, Highly Sensitive Micro HPLC Method fot the Determination of Coenzyme Q10 and its Major Related Substances
Autor/es:
TRIPODI V.; FLOR S; CONTIN M.; LUCANGIOLI S.
Revista:
JOURNAL OF LIQUID CHROMATOGRAPHY AND RELATED TECHNOLOGIES
Editorial:
TAYLOR & FRANCIS INC
Referencias:
Lugar: BOCA RATON; Año: 2009 vol. 32 p. 860 - 873
ISSN:
1082-6076
Resumen:
Abstract: A simiple, rapid, and highly sensitive fast microbore HPLC method with UV detection has been developed for the quantitation of coenzyme Q10 (Co-Q10) and related substances in raw material, pharmaceutical, and cosmetic formulations using a column with hybrid particles. Chromatographic conditions were: 30ºC column temperature, 1 mL injection volume at 275 nm. The Co-Q10 analysis was performed within 2.5minutes with a flow rate of 0.3mL=min. with a mobile phase consisted of 100% methanol, and the related substances were separated with a gradient elution based on methanol-water. The developed method was validated for selectivity, linearity, precision, accuracy, and robutness. The method was found to be suitable for the quality control of Co-Q10 in pharmaceutical and cosmetic products, as well as the stability indicating studies.A simiple, rapid, and highly sensitive fast microbore HPLC method with UV detection has been developed for the quantitation of coenzyme Q10 (Co-Q10) and related substances in raw material, pharmaceutical, and cosmetic formulations using a column with hybrid particles. Chromatographic conditions were: 30ºC column temperature, 1 mL injection volume at 275 nm. The Co-Q10 analysis was performed within 2.5minutes with a flow rate of 0.3mL=min. with a mobile phase consisted of 100% methanol, and the related substances were separated with a gradient elution based on methanol-water. The developed method was validated for selectivity, linearity, precision, accuracy, and robutness. The method was found to be suitable for the quality control of Co-Q10 in pharmaceutical and cosmetic products, as well as the stability indicating studies.mL injection volume at 275 nm. The Co-Q10 analysis was performed within 2.5minutes with a flow rate of 0.3mL=min. with a mobile phase consisted of 100% methanol, and the related substances were separated with a gradient elution based on methanol-water. The developed method was validated for selectivity, linearity, precision, accuracy, and robutness. The method was found to be suitable for the quality control of Co-Q10 in pharmaceutical and cosmetic products, as well as the stability indicating studies.=min. with a mobile phase consisted of 100% methanol, and the related substances were separated with a gradient elution based on methanol-water. The developed method was validated for selectivity, linearity, precision, accuracy, and robutness. The method was found to be suitable for the quality control of Co-Q10 in pharmaceutical and cosmetic products, as well as the stability indicating studies.