Expresión de proteínas inducibles por frío en la médula espinal de rata sometida a hipotermia sistémica

SAROTO AJ, REY-FUNES M, DORFMAN VB, CONTARTESE D, LARRÁYOZ IM, MARTÍNEZ A, TOSCANINI MA; LOIDL CF

Revista de la Asociación Argentina de Ortopedia y Traumatología

Asociación Argentina de Ortopedia y Traumatología

Año: 2022 vol. 87 p. 393 - 403

1852-7434

Introduction: Traumatic spinal cord injury is the main cause of motor disability in developed and underdeveloped countries, beinga priority interest to the WHO. The effect of hypothermia on the expression of CIRBP (cold-activated protein) in the anterior greycolumn of 60-day-old male albino Sprague-Dawley rats was studied at the structural and biochemical levels and proposed as apossible therapeutic approach. Materials and Methods: 24 rats were randomly divided into two groups; normothermia (n = 6),at 24° C, and hypothermia, (n = 18) at 8° C for 180 minutes and euthanized at 12, 24, and 48 h post-treatment. Western blot andimmunohistochemistry for CIRBP were used. Results: A progressive increase in the expression of CIRBP was observed from 12to 48 hours, with statistically significant values after 24 and 48 hours compared to controls. Conclusion: This experimental modeldemonstrated efficacy, accessibility, and economy to generate systemic hypothermia, which provides a novel range of therapeuticstrategies. The increase in the expression of cold-inducible proteins in the rats’ spinal cords allows us to study the benefit of hypothermiaat the molecular level for the first time, being of utmost importance for therapeutic studies in spinal cord injuries.