Analysis of Expression Gradients of Developmental Regulators in Arabidopsis thaliana Roots

ERCOLI, MARÍA FLORENCIA; VENA, RODRIGO; GOLDY, CAMILA; PALATNIK, JAVIER F.; RODRÍGUEZ, RAMIRO E.

METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J.)

Humana Press Inc.

Año: 2018 vol. 1863 p. 3 - 17

1064-3745

The regulatory mechanisms involved in plant development include many signals, some of them acting as graded positional cues regulating gene expression in a concentration-dependent manner. These regulatory molecules, that can be considered similar to animal morphogens, control cell behavior in developing organs. A suitable experimental approach to study expression gradients in plants is quantitative laser scanning confocal microscopy (LSCM) using Arabidopsis thaliana root tips as a model system. In this chapter, we outline a detailed method for image acquisition using LSCM, including detailed microscope settings and image analysis using FIJI as software platform.