Antibodies from healthy or paratuberculosis infected cows have different effects on Mycobacterium avium subspecies paratuberculosis invasion in a calf ileal loop model

JOLLY, ANA; FERNÁNDEZ, BÁRBARA; STEMPLER, ANA; INGRATTA, GISELLE; POSTMA, GABRIELA; BOVIEZ, JUAN; LOMBARDO, DANIEL; HAJOS, SILVIA; MUNDO, SILVIA L.

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY

ELSEVIER SCIENCE BV

Año: 2022 vol. 245 p. 1 - 8

0165-2427

In this work, we used a calf ileal loop model to evaluate whether the preincubation of Mycobacterium avium subspecies paratuberculosis (MAP) with antibodies from healthy, MAP-positive or Lipoarabinomannan (LAM) immunized cows could affect the results of infection after 3.5 h. Bacterial load in tissue was assessed by Ziehl- Neelsen and by culture for each loop. MAP was detectable in all infected loops after 3.5 h.p.i.; although the presence of antibodies from MAP-positive cows significantly reduced bacterial load in loops as compared with antibodies from healthy donors (by Ziehl-Neelsen and culture, p-value