Protein adsorption onto tentacle cation-exchange hollow-fiber membranes

CAMPERI, SILVIA A., NAVARRO DEL CAñIZO, AGUSTíN A., WOLMAN, FEDERICO J., SMOLKO, EDUARDO E., CASCONE OSVALDO, GRASSELLI, MARIANO

BIOTECHNOLOGY PROGRESS

American Chemical Society and American Institute of Chemical Engineers

Lugar: Washington, USA; Año: 1999 vol. 15 p. 500 - 505

8756-7938

A sulfonic group (up to 200 ìmol/mL membrane) was incorporated to epoxy-activated microporous hollow fibers to obtain high-capacity convective ion exchangers. The pure water flux through the membrane decreased exponentially with sulfonic group density and protein binding capacity incresed accordingly. At sulfonic group density of 70 ìmol/mL, the membrane lysozyme maximum binding capcity was 84 ± 9 mg/mL in comparison with its theorical monolayer maximum binding capacity of 20 mg/mL, thus evidencing tentacle formation. After a cycle of adsorption in a 30 mM sodium phosphate buffer, pH 7.0, adsorbed lysozyme could be quantitatively recovered following elution with 0.5 M NaCl in the same buffer. Dynamic capacity for lysozyme was 67% of maximum binding, and its value did not change at space velocities ranging from 10 to 40 min-1 as shown by the superimposition of the corresponding breakthrough curves. A cartridge assembled with 21 fibers showed a dynamic-to-static capacity ratio for lysozyme of 0.60 with 1 mg/mL pure lysozyme solution, and 0.42 with a particulate feed composed of 1 mg/mL lysozyme and 0.1 mg/mL yeast.