Influence of superparamagnetic nanoparticles in chitin structure and lysozyme adsorptive properties

GABRIEL I. TOVAR-JIMENEZ; ROBERTO FERNÁNDEZ DE LUIS; MARÍA ISABEL ARRIOURTUA; FEDERICO JAVIER WOLMAN; GUILLERMO JAVIER COPELLO

Congreso virtual de química a través de Twitter

Congreso; LatinXChem; 2020

Royal Society of Chemistry

Lysozyme (LYZ) is a protein of great importance in the food industry because of its antibacterial activity. Thus, its separation and purification has long been a matter of study. In this work, a novel nanocomposite for lysozyme purification was obtained using chitin for the wrapping of superparamagnetic nanoparticles (MNPs).Method The nanocomposite synthesis process was simple and scalable and did not alter the structure and magnetic properties of MNPs (70emu/g). The XRD patterns were adjusted with a Le-Bail profile matching. The incorporation of MNPs, not only altered the macroscopic structure of chitin polymer, also induces a slight reorganization of the polysaccharide chains.ResultTo characterize the magnetic composite´s interaction with LYZ, the effect of pH on the absorptive capacities and kinetic parameters was examined. The results indicated that the nanocomposite presents an adsorption capacity of 488mg/g of lysozyme at pH 9.We can recover LYZ without diluting or pretreating the hen egg-white, leading to a 75% global yield and a purity degree >99%.ConclusionThe MNPs influence the structure of the chitin support by increasing of tenfold the surface area from the chitin gel to the nanocomposite.