WOLMAN Federico Javier
congresos y reuniones científicas
Purificación de lactoferrina de calostro bovino por cromatografía de afinidad sobre membranas
F. J. WOLMAN, E. E. SMOLKO, M. GRASSELLI, O. CASCONE.
San Miguel de Tucumán, provincia de Tucumán, Argentina
Congreso; Simposio Internacional de Biotecnología. Aplicaciones en Alimentos, Salud y Medio Ambiente. II Simposio Argentino-Italiano de Bacterias Lácticas.; 2004
Microfiltration hollow-fibre membranes were grafted with glycidyl methacrylate (membranes I) or a mixture of glycidyl methacrylate and dimethyl acrylamide 1:2.1, v/v (membranes II) and Red HE-3B was covalently attached to them after reaction of the grafted epoxy groups with ammonia. Membranes permeability was 37.0 and 7.1 ml/cm2. min.bar for membranes I and II respectively. A maximum capacity of 21.5 and 110.2 mg/ml and dissociation constants of 0.08 and 0.75 mg/ml were calculated from the adsorption isotherms for membranes I and II respectively. Different eluents were assayed, and 2 M NaCl + 25 % ethylene glycol was chosen, eluting 90 % of lactoferrin adsorbed on membranes II and only 15.8 % of lactoferrin adsorbed on membranes I. Membranes II-Red HE-3B were selected for lactoferrin purification from colostrum due to their higher capacity and elution percentage . Performance of membranes II-Red HE-3B was compared with that of Sepharose 4B-Red HE-3B, resulting in a 97 % higher recovery of electrophoretically pure lactoferrin.