One-step lactoferrin purification from bovine whey and colostrum by affinity membrane chromatography.

F. J. WOLMAN, D. GONZÁLEZ MAGLIO, M. GRASSELLI, O. CASCONE

JOURNAL OF MEMBRANE SCIENCE

Elsevier

Lugar: Amsterdan; Año: 2007 vol. 288 p. 132 - 138

0376-7388

Abstract  Hollow fibres with high lactoferrin (Lf) adsorption capacity were synthesised by grafting a glycidyl methacrylate/dimethyl acrylamide copolymer to polysulfone membranes and attaching the dye Red HE-3B to them.A maximum capacity of 111.0 mg Lf ml-1 membrane , far higher than that obtained with the same ligand immobilised on agarose beads (9.3 mg ml-1), was found from isotherms developed with Lf aqueous solutions.With colostrum as the Lf source in a batch system, 91 % of the Lf contained in that raw material was adsorbed by the dye-membranes while only 56 % was adsorbed by the dye-agarose beads.The best desorption performance was achieved with 2 M NaCl in 25 % ethylene glycol: 99 % and 80 % from the hollow fibres and agarose beads, respectively.A cartridge assembled with dye hollow fibres showed a productivity of 501.5 µg h-1 ml-1 Lf, 500 % higher than that achieved with the dye agarose matrix.A 94 % purity was achieved in only one step, being casein and immunoglobulin the only contaminants. As Lf is usually utilised as a food supplement, Lf obtained by the method described comply with the needs of a final product. Key words: Lactoferrin, Purification, Whey, Colostrum, Dye-affinity membranes. Key words: Lactoferrin, Purification, Whey, Colostrum, Dye-affinity membranes. Key words: Lactoferrin, Purification, Whey, Colostrum, Dye-affinity membranes. Key words: Lactoferrin, Purification, Whey, Colostrum, Dye-affinity membranes. Key words: Lactoferrin, Purification, Whey, Colostrum, Dye-affinity membranes.