INVESTIGADORES
WOLMAN Federico Javier
artículos
Título:
Affinity chromatography with pseudobiospecific ligands on high-performance supports for purification of proteins of biotechnological interest
Autor/es:
IANNUCCI, NANCY B., WOLMAN, FEDERICO J., CAMPERI, SILVIA A., NAVARRO DEL CAÑIZO, AGUSTÍN A., GRASSELLI, MARIANO, CASCONE OSVALDO
Revista:
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
Editorial:
Asociación Brasilera de Ingeniería Química
Referencias:
Lugar: San Pablo, Brasil; Año: 2003 vol. 20 p. 27 - 27
ISSN:
0104-6632
Resumen:
High-performance affinity matrices were obtained by attaching pseudobiospecific ligands to hollow-fibre membranes. The neutral protease contained in FlavourzymeTM was purified to homogeneity with Yellow 4R-HE affinity hollow-fibre membranes. Immobilisation of Red HE-3B allowed purification of a milk-clotting enzyme obtained by solid-state culture of Mucor bacilliformis. Cooper immobilisation through iminodiacetic acid allowed fractionation of Biocon Bioconcentrated PlusTM to separate the pectinesterase-containing fraction. The productivity of the developed processes – 1900, 94 and 750 U/ml.min, respectively – was 10-to 15-fold higher than that achieved with the same ligands immobilised on agarose-based soft gels, mainly due to the shortening of the purification processes