WOLMAN Federico Javier
capítulos de libros
Strategies to recover and purify lysozyme from egg white
FEDERICO JAVIER WOLMAN; MARÍA FERNANDA BAIELI; NICOLÁS URTASUN; AGUSTÍN A. NAVARRO DEL CAÑIZO; MARÍA VICTORIA MIRANDA; OSVALDO CASCONE
Lysozymes: Sources, Functions and Role in Disease
Nova Science Publishers Inc
Lugar: Hauppauge, New York; Año: 2013; p. 241 - 250
The present article analyzes different strategies to purify lysozyme from hen egg white. Since its discovery in 1922, new strategies have arisen to purify lysozyme from hen egg white. However, most of these strategies are based on the use of diluted egg white as the starting material, which has impaired its commercialization path for confectionery. Only a few processes use egg white without any conditioning or pretreatment, which accounts for the difficulty in the egg white processing; however, taking into account the large market for this enzyme and its high concentration in egg white, it is more convenient to obtain lysozyme from its natural source than from recombinant expression. Here, the different approaches for lysozyme purification are analyzed taking into account the protein-ligand interaction ?ionic exchange chromatography, pseudobioaffinity to triazinic dyes and affinity to N-acetyl glucosamine polymers- and considering the chromatographic support material or matrix for an adequate egg white processing.