INVESTIGADORES
VIDAL RUSSELL Romina
congresos y reuniones científicas
Título:
Higher-order structure of nuclear large-subunit rDNA from Moquiniella rubra (Loranthaceae)
Autor/es:
BONE, T.; VIDAL-RUSSELL, R.; NICKRENT, D. L.
Lugar:
Charleston, Illinois, EE.UU.
Reunión:
Congreso; Illinois State Academy of Sciences Annual Meeting; 2004
Institución organizadora:
Illinois State Academy of Sciences
Resumen:
Loranthaceae is the largest family in the mainly parasitic order Santalales. Large-subunit (LSU) rRNA is currently being used in an extensive study whose goal is to determine the phylogeny for the entire family Loranthaceae. LSU rRNA has been used in molecular phylogenetic studies of all angiosperms, but has only rarely been used at the family level but has been found to provide sufficient phylogenetic signal to resolve relationships among loranth genera. To aid in alignment, structural features should be incorporated, thus ca. 2000 bp of LSU rRNA were determined for Moquiniella rubra. Length of whole molecule has conserved and variable domains (D12). A higher-order structure for the 5’ 1883 bp M. rubra LSU rRNA was constructed encompassing variable regions D1-D7. The Gutell-Noller-Woese model of Lycopersicon LSU rRNA was used to prepare a structure for Citrus, a species more closely related to Moquiniella and this structure was then used as a guide. The Moquiniella structure was similar in all major features to that of Citrus. Pairwise comparisons revealed 108 substitutional changes (5.7%), 86 of which occurred in variable domains D1-D7. 63 of these were transitions and 44 transversions. 36 substitutional mutations occurred in paired (stem) regions and 72 were in unpaired (loop) regions.