Antiviral potential of extracts from Myriogloea major against herpes simplex type 1, 2 and bovine coronavirus

CONESA, ANA LUCÍA; DELLATORRE, FERNANDO GASPAR; LATOUR, EZEQUIEL; PONCE, NORA MARTA ANDREA; STORTZ, CARLOS A.; SCOLARO, LUIS ALBERTO; ÁLVAREZ, VERA ALEJANDRA; LASSALLE, VERÓNICA LETICIA; AYALA?PEÑA, VICTORIA BELEN

PHYCOLOGICAL RESEARCH (PRINT)

WILEY-BLACKWELL PUBLISHING, INC

Año: 2024

1322-0829

Fucose-rich sulfated polysaccharides such as fucoidans areprimarily found in brown algae. These compounds possessinteresting pharmacological perspectives for antiviral and antioxidantdrug development. The brown alga Myriogloea major(Chordariaceae, Ectocarpales) is an endemic brown alga fromArgentine Patagonia similar to other members of the Chordariaceaefamily used as a commercial source of fucoidans.In the present study, M. major was extracted with dilutedhydrochloric acid (pH 2) solution in a protocol usually utilizedfor obtaining fucoidans. The dry weight from two extracts atdifferent extraction stages (E1 and E2) represented 42% ofthe extracted dried biomass. Chemical analysis showed thatthe extracts contained between 15% (E2) and 20% (E1) ofsulfate esters and approximately 80% of fucose constituentson their polysaccharides. The total phenolic content was lowerthan 1% (gallic acid equivalents). We analyzed the antioxidantactivity of these extracts against the anti-herpes simplexvirus (HSV)-1, HSV-2 and bovine coronavirus. The antioxidantactivity was lower than 50% at a concentration of 1 mg mL1in both extracts. Our findings demonstrate that, in the presenceof these extracts, the viral adsorption and internalizationwere reduced by up to 75%. By applying its antiviral action toblock the viral infection, the progeny virus released from hostcells was significantly restricted, without affecting cell-to-cellspread of virus or virus viability.