Isolation and Characterization of Microsatellite Markers for Hypochaeris incana (Asteraceae) and Close Relatives

PING WANG; KARIN TREMETSBERGER; ESTRELLA URTUBEY; KARL-GEORG BERNHARDT

Applications in Plant Sciences

Botanical Society of America

Lugar: Washington; Año: 2017 vol. 5 p. 1 - 4

? Premise of the study: We developed microsatellite markers to study clonal growth and interspecific hybridization in the Patagonianand subantarctic plant Hypochaeris incana (Asteraceae) and its closest relatives.? Methods and Results: We developed primers for microsatellite loci from 454 sequence reads of genomic DNA of H. incana. Wetested them on individuals of H. acaulis, H. hookeri, H. incana, H. palustris, and H. tenuifolia. We selected 15 polymorphicmicrosatellite loci, which delivered clearly scorable fragments in most or all species. With mean values between 0.7 and 0.8, theexpected heterozygosity in populations of H. incana is high.? Conclusions: Due to high levels of polymorphism, the developed markers make it possible to distinguish between genets andramets in H. incana. In some markers, null alleles complicate the scoring of genotypes in tetraploids. All of the developed markersare suitable to study interspecific hybridization among this group of closely related species.