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Título:
Neurorestorative and protective effects of palmitoylethanolamide in perinatal asphyxia: an analysis of the rat striatum
Autor/es:
LUCAS D. UDOVIN; TAMARA KOBIEK; MARÍA I. HERRERA; ANA B. RAMOS HRYB; NICOLAS TORO; CARLOS KUSNIER; FRANCISCO CAPANI
Reunión:
Congreso; XXXIII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; 2018
Resumen:
Perinatalasphyxia(PA)causedbylowO2availabilityduringbirthisassociatedwithbraindamage,beenthestriatumoneofthemostaffectedareas.Palmitoylethanolamide(PEA)isaneuroprotectiveamideinbraininjurymodels,includingPA.However,itseffectsagainstPArequiresdeeperstudyinthestriatum.UsingBjelke?model,full-termpregnantratswererapidlydecapitated,anduterushornsplacedinawaterbath(37°C,19min).Onehourafter,ratsweretreatedwithPEA(10mg/kg,s.c.)andlatergiventosurrogatemothers.Thirtydayanimalswereperfusedandstriatumwasanalysedeitherforimmunohistochemistry(n=3)andblotting(n=3).Phosphorylatedhigh/mediummolecularweightneurofilaments(pNF-H/M),Microtubule-AssociatedProtein2(MAP2),andGlialfibrillaryacidicprotein(GFAP)wereanalysed.2-wayANOVAfollowedbyTukeyanalysisrevealedareductionin:pNF-H/MandMAP2reactiveareas(45%and70%),GFAP+cells(20%)andreducedMAP2andpNF-H/MproteinlevelsinPA-ratstriatumrespectivelytothecontrolvalue.PEAtotallyrestoredGFAP+cellsandMAP2immunoreactionandpartiallypreventedthedecreasedpNF-H/Mreactivearea(78%)inducedbyPA.PEAalsoreversedtheMAP2reductionandpartiallypreventedthedecreaseofpNF-H/MproteinlevelsinducedbyPA.NoalterationintheGFAPproteinlevelswasdetected.PEAtreatmentattenuatedthestriatumdamageinducedbyPA,demonstratingitstherapeuticpotentialagainstPA.