PERSONAL DE APOYO
UDOVIN lucas
congresos y reuniones científicas
Título:
NEURODEGENERATIVE MODIFICATIONS DRURING PERINATAL ASPHYXIA: CORRELATIVE LIGHT AND ELECTRON MICROSCOPY STUDIES
Autor/es:
FRANCISCO CAPANI , MARIA INÉS HERRERA, LUCAS UDOVIN , CARLOS F. KUSNIER , NICOLÁS TORO , CECILIA QUARRACINO , TAMARA KUBIEC , RODOLFO KOLLIKER FRERS , JUAN PABLO LUACES
Lugar:
Sydney
Reunión:
Congreso; 19th International Microscopy Congress 9 - 14 September 2018 International Convention Centre, Sydney; 2018
Resumen:
NEURODEGENERATIVE MODIFICATIONS DRURING PERINATAL ASPHYXIA: CORRELATIVE LIGHT AND ELECTRON MICROSCOPY STUDIES.Francisco Capani (1 y 3), Maria Inés Herrera (1 y 2), Lucas Udovin (1),), Carlos F. Kusnier (1), Nicolás Toro (1), Cecilia Quarracino (1), Tamara Kubiec (2), Rodolfo Kolliker Frers (1),.Juan Pablo Luaces (4)(1) Instituto de Investigaciones Cardiológicas (UBA-CONICET), UBA-CONICET, Buenos Aires, Argentina; (2) CIPP Universidad Católica Argentina. (3) Departamento de Biología,UAJFKStatement of the Problem: Diminish in the oxygen levels prompted short and long-term alterations in synapses and related structures that are related to neuronal dysfunction and death. Perinatalasphyxia (PA) is an obstetric complication produced by an impaired gas exchange that lead to neonatal mortality and is a determinant factor for neurodevelopmental disorders. Since pathophysiologicalmechanisms triggered by PA are not still totally unveiled, we investigated the changes in the cytoskeleton organization in the nervous tissue. Methodology & Theoretical Orientation: For this study,we used a well-established murine model of PA [1]. After one, 2, 4 and 6 months of severe PA (20 min) rats were sacrificed and their brains were analyzed by combining photooxidation, conventionalelectron microscopy, and 3-D reconstruction techniques [1]. Findings: After one month of PA, we found an increase in the F-actin staining in neostriatal and hippocampal dendritic spines together withsome filopodia-likes structures, a typical embryonic type of spines in photooxidated tissue [2] [Fig 1 A). In contrast, after second month of PA, spines were less consistent stained. In addition, weobserved an increment of marker for neuronal and glial dysfunction such as GFAP, neurofilament and MAP-2 [4,5]. These modifications were more striking defined after 4 months of PA [3,4]. After 6months of PA post-synaptic densities (PSDs) in neostriatum were highly modified. Using three-D reconstructions and electron tomography we were able to find clear signs of degeneration in theasphyctic PSDs (Fig 1 B and C) [1] Conclusion & Significance: Therefore, we hypothesize that the cytoskeletal changes induced by PA in the rat CNS could lead to the dramatic modifications insynapse and related structures that trigger neuronal damage. In addition, electron tomography, 3-D reconstruction and photooxidation contributed to dissect critical alterations generated by PA thatare not easily displayed using conventional microscopic techniques.