INVESTIGADORES
TORIANO Roxana Mabel
artículos
Título:
Water flux through human aquaporin 1: inhibition by intracellular furosemide and maximal response with high osmotic gradients
Autor/es:
MARCELO OZU; RICARDO A. DORR; M. TERESA POLITI; MARIO PARISI; ROXANA TORIANO
Revista:
European Biophysical Journal
Editorial:
SPRINGER
Referencias:
Lugar: European Biophysics Journal ; Año: 2011 vol. 40 p. 737 - 746
ISSN:
1432-1017
Resumen:
This work studies water permeability properties of human aquaporin 1 (hAQP1) expressed in Xenopus laevis oocyte membranes, applying a technique where cellular content is replaced with a known medium, with the possibility of measuring intracellular pressure. Consequences on water transport—produced by well-known anisotonic gradients and by the intracellular effect of probable aquaporin inhibitors—were tested. In this way, the specific intracellular inhibition of hAQP1 by the diuretic drug furosemide was demonstrated. In addition, experiments imposing anisotonic mannitol gradients with a constant ionic strength showed that the relationship between water flux and the applied mannitol gradient deflects from a perfect osmometer response when the gradient is higher than 150 mosmol kgW -1. These results would indicate that the passage of water molecules through hAQP1 may have a maximum rate. As a whole, this work demonstrates the technical advantage of controlling both intracellular pressure and medium composition in order to study biophysical properties of hAQP1, and contributes information on water channel behavior under osmotic challenges and the discovery of new inhibitors.