Diversity of Phaeoisariopsis griseola populations isolated from Argentina

STENGLEIN S.A., FERMOSELLE G.E., PLOPER L.D., VIZGARRA N.O. & BALATTI P.A.

Carlos Paz, Cordoba

Congreso; XIII Congreso Latinoamericano de Fitopatología y III Taller Argentino de Fitopatología; 2005

Angular Leaf Spot (ALS), a disease of common bean (Phaseolus vulgaris L.), is caused by an imperfect fungus known as Phaeoisariopsis griseola (Sacc.) Ferraris. We analyze, based on virulence and molecular markers, the variability among 45 argentinian isolates of P. griseola collected from Northwestern Argentina. Pathogenicity of the isolates was assessed on a set of 12 bean differentials and a wild bean, finding thirteen pathotypes. The wild bean turned out to be a good source of resistance to P. griseola. Seven Random Amplified Polimorphic DNA (RAPD) and Inter-Simple Sequence Repeats (ISSR) primers yielded consistent polymorphic banding patterns and were used to analyze molecular diversity. Both types of markers clustered isolates of P. griseola as Mesoamerican or Andean.