INVESTIGADORES
SOMOZA Gustavo Manuel
artículos
Título:
Analysis of sexually dimorphic expression of genes at early gonadogenesis of pejerrey Odontesthes bonariensis using a heterologous microarray
Autor/es:
JUAN I. FERNANDINO; JASON T. POPESKU; BINDHU PAUL-PRASANTH; HUILING XIONG; RICARDO S. HATTORI; MIHO OURA; CARLOS A. STRÜSSMANN; GUSTAVO M. SOMOZA; MASARU MATSUDA; YOSHITAKA NAGAHAMA; VANCE L. TRUDEAU
Revista:
SEXUAL DEVELOPMENT
Editorial:
KARGER
Referencias:
Año: 2011 vol. 5 p. 89 - 101
ISSN:
1661-5425
Resumen:
The process of morphological development of a differentiated gonad from anundifferentiated primordium is a very important step of gonadogenesis. Studies on sexuallydimorphic gene expression are important to increase our understanding of this process and toinvestigate how environmental factors such as temperature can regulate gonadal development.The aim of this study was to identify putative genes involved in sex differentiation in pejerrey(Odontesthes bonariensis) reared at male- and female-producing temperatures (MPT and FPT,respectively) using a microarray heterologous from the medaka (Oryzias latipes), a relatedspecies. Genes related to numerous processes presented higher expression at MPT, includingthose involved in muscular contraction, metabolic pathways, developmental processes, andreproduction. Genes induced by FPT were classified under the gene ontology terms of responseto stimulus, transport and proteolysis. From genes selected for validation, at MPT ndrg3expression was observed in the somatic cells, whereas pen-2 was detected in germ cells in thecaudal portion of the gonads, where no apoptotic signals was detected. Finally, hsp90 was highlyexpressed in somatic cells of the gonads at the FPT. The results suggest that the interplay of proapoptoticand anti-apoptotic genes is important during the masculinization process and for theprevention of sterility following exposure to warm temperatures.