INVESTIGADORES
SOMOZA Gustavo Manuel
artículos
Título:
Identification of immunoreactive Gonadotropin-Releasing Hormone (mGnRH) in the brain of metamorphic larvae of Bufo arenarum Hensel (Amphibia: Anura).
Autor/es:
GUSTAVO M SOMOZA; DANTE PAZ; ANDREA STEFANO; JORGE AFFANNI; GUSTAVO M SOMOZA
Revista:
INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Año: 1996 vol. 14 p. 663 - 672
ISSN:
0736-5748
Resumen:
Gonadotropin!releasing hormone GnRH immunoreactivity in brain extracts of Bufo arenarumtadpoles were investigated by high!performance liquid chromatography\ followed by radioimmunoassay analysis using two different antisera raised against different GnRH variants. Only one immunoreactive peak was identified eluting in the same position as synthetic mammalian GnRH. This result was further confirmed by serial dilution studies using more speciffic mammalian GnRH antisera. Our results suggest that mammalian GnRH is most likely an endogenous peptide in the brain of the developing larvae and froglets of Bufo arenarum and quite likely it is the only GnRH variant present during those development stages. The distribution and density of cell bodies and fibers were analysed by immunocytochemical procedures. Immunoreactive cell bodies appeared in the olfactory epithelium and across the olfactory nerve at late prometamorphic larval stages. Near the metamorphic climax and in froglets, perikarya and fiberswere detected in basal forebrain, preoptic and hypothalamic areas. No immunoreaction was observed at midbrain, hindbrain and spinal cord levels. This study suggests that mammalian GnRH is most likely an endogenous peptide and is probably the only GnRH variant in the brain of the developing larvae and froglets of Bufo arenarum.