INVESTIGADORES
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artículos
Título:
Analytical tools for elucidating the biological role of melatonin in plants by liquid chromatography tandem mass spectrometry
Autor/es:
F. GÓMEZ, I. GATICA, L. MARTINEZ, M.F. SILVA, S. CERUTTI
Revista:
ELECTROPHORESIS
Editorial:
WILEY-V C H VERLAG GMBH
Referencias:
Lugar: Weinheim; Año: 2013
ISSN:
0173-0835
Resumen:
Melatonin (MT) presence in higher plants was recently discovered and the
knowledge of its function in vivo is limited. Several studies have
recently shown the occurrence of MT and related compounds in grapes and
wines. The analysis of MT in plants and foods represents a highly
challenging task due to its wide concentration range, the difficulty in
the selection of the extraction solvents because of its amphipathic
nature, and the fact that it reacts quickly with other matrix
components. Thus, sample processing factors; preparation/cleanup
procedures; and chromatographic/detection parameters, such as HILIC and
reverse phase (C8 and C18) chromatographic modes,
ESI, and atmospheric pressure chemical ionization (APCI) in both
negative and positive modes were evaluated. Taken together, we have
demonstrated that optimal conditions were quite different for each of
the matrices under study. A sonication-mediated extraction step was
necessary for grape skin (100% v/v methanol) and plant tissues (50% v/v
methanol), while wine and must required a SPE preconcentration step.
HILIC−(+) APCI ionization was better for MT standards, while C8−(+) APCI was the best choice for grape skin and C18−(+ESI) was suitable for wine. On the other hand, C8−(+)ESI was the most appropriate for vegetal tissues of Arabidopsis thaliana.
Proposed methods were validated and the LODs were in the low picogram
levels range. The optimized approaches were applied to the determination
of MT and its isomer in different vegetal/food samples; levels found
within the range: 4.9440 ng/g.