Interaction effects between maternal hyperthyroidism and prenatal stress on male wistar offspring postnatal development and locomotor activity

MICHEL LARA, MC; SANCHEZ MB; NEIRA FJ; VIRUEL, L.B.; PIETROBON EO; SOAJE M; GOMEZ S; ACCORINTI C; JAHN GA; MACKERN-OBERTI JP; VALDEZ SR

San Juan

Congreso; XLI Reunión Anual de la Sociedad de Biología de Cuyo; 2023

Sociedad de Biología de Cuyo

EFFECTS ON POSTNATAL DEVELOPMENT AND LOCOMOTOR ACTIVITY OF MALE WISTAR OFFSPRING OF THE INTERACTION BETWEEN MATERNAL HYPERTHYROIDISM AND PRENATAL STRESSMichel Lara MC1,2, Sánchez MB1,2, Neira FJ1,2, Viruel LB1,2, Pietrobon EO1,4, Soaje M1,5, Gomez SE1, Accorinti C1, Jahn GA1, Mackern-Oberti JP1,5 and Valdez SR1,61Instituto de Medicina y Biología Experimental de Cuyo, CONICET, UNCuyo, 2Universidad Juan Agustín Maza, 3Instituto de Histología y Embriología de Mendoza, UNCuyo, 4Instituto de Fisiología, UNCuyo, 5Facultad de Ciencias Exactas y Naturales, UNCuyo. Mendoza, Argentina. cemichel@outlook.com Thyroid hormones (THs) [triiodothyronine (T3) and thyroxine (T4)] are essential for various physiological functions and can cross the placenta during pregnancy to play a vital role in fetal development. Hyperthyroidism (HyperT) results from excessive THs production, which can lead to several maternal, fetal, and lactational complications. Prenatal stress (PS) can alter the histoarchitecture and functionality of the offspring´s brain, increasing the risk of neurodevelopmental disorders and mental illness. Moreover, PS can also impact THs and corticosterone (C, stress hormone in rats) levels, affecting reproductive health. Our objective was to determine the effects of the interaction between HyperT and PS on male offspring development. Pregnant Wistar rats were divided into four groups: control (euthyroid, Co), HyperT (administration of T4, 0.1 mg/kg/day dose, subcutaneous, throughout the protocol), Chronic Unpredictable Moderate Stress (CUMS), and HyperT+CUMS. CUMS was applied from gestational days 6 to 17. Male offspring were kept in standard conditions, and their developmental parameters were assessed from birth to postnatal day (PD) 42. The Open Field Test (OFT) was used to analyze their exploratory and locomotor activity at two points: young (PD24) and adults (PD85). Trunk blood and adrenal glands (AG) were collected on PD110. One-way ANOVA statistical analysis was performed. The results showed that offspring exposed to HyperT+CUMS exhibited reduced size at PD1, as evidenced by lower body weight (p