NEURODEVELOPMENTAL ALTERATIONS IN FEMALE WISTAR RATS: LONG TERM-EFFECTS OF MATERNAL HYPERTHYROIDISM AND PRENATAL STRESS

MICHEL LARA, MC; SANCHEZ MB; NEIRA FJ; VIRUEL, L.B.; PITTON J; PIETROBON EO; SOAJE M; JAHN GA; MACKERN-OBERTI JP; VALDEZ SR

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

Sociedad Argentina de Investigación Clinica

Thyroid hormones (THs) [thyroxine (T4) and triiodothyronine (T3)]play vital roles in physiological functions and are crucial for normalfetal brain development. THs can pass from mother to fetus duringgestation. Hyperthyroidism (HyperT), resulting from excessive THproduction, can lead to maternal, fetal, and lactational complicationsduring pregnancy. Prenatal stress (PS) has been linked to changesin the structure and function of offspring brains, potentially contributing to neurodevelopmental and mental disorders. PS can also affect thyroid hormone and corticosterone (C, stress hormone in rats)levels, impacting reproductive health. We studied the long-term effects of the interaction between HyperT and PS on female offspringdevelopment. Maternal Wistar rats were divided into four groups:control (normal thyroid, Co), HyperT (administration of T4, 0.1 mg/kg/day doses, subcutaneous, throughout the protocol), Chronic Unpredictable Moderate Stress (CUMS), and HyperT+CUMS. CUMSwas applied from gestational days 6 to 17. Female offspring wereraised in standard conditions, and developmental parameters wereassessed from birth to postnatal day (PD) 42. At adulthood (PD85-100), behavioral tests (OFT, EPM, FST) were conducted to evaluatethe locomotor activity and anxious-depressive states. Truncal bloodand adrenal gland (AG) histology were collected on PD110. Oneway ANOVA statistical analysis was performed. Interaction betweenHyperT and PS led to reduced birth weight (p