INVESTIGADORES
D`ALESSIO Luciana
congresos y reuniones científicas
Título:
Nestin inmunorreactivity in dentate gyrus of patients with hippocampal sclerosis.
Autor/es:
D' ALESSIO L, KONOPKA H, ESCOBAR E , GORI MB, SOLÍS P, SEOANE E, KOCHEN S.
Lugar:
Buenos Aires
Reunión:
Congreso; VIII Congreso Latinoamericano de Epilepsia; 2014
Institución organizadora:
LIiga Internacional contra la Epilepsia (ILAE)
Resumen:
Nestin is a protein expressed by neural progenitor cells (NPCs) and was used to label a putative neurogenic activity in adult brain. Nestin expression in dentate gyrus (DG) indentifies proliferative neurons and new generated cells. The aim of this study was to determine the nestin inmunoreactivity in hippocampal tissue obtained from temporal lobe epilepsy (TLE) in patients with hippocampal sclerosis (HS) who underwent epilepsy surgery. Methods Hippocampal sections of 16 patients with HS and resistant TLE who underwent epilepsy surgery, were processed using immunoperoxidase for Nestin. Archival material from 8 normal post-mortem hippocampus, were simultaneously processed. Reactive área for nestin, the total number of positive nestin cells (NC) per field, and MGV (mean gray value) was determined by computarized image analysis (image J), and compared between groups. Student t test was used for statistical analysis. Results In both, control and epileptic hippocampus NC were found in different areas including, DG, pyramindal layers (CA1-CA4) and subpial zones (SZ). In epileptic tissue, NC in the DG showed cell dispersion, ectopic localization, and larger somas and reactive areas p