INVESTIGADORES
SELVA juan pablo
congresos y reuniones científicas
Título:
Epigenetic Mechanisms Involved in Diplospory Regulation in Weeping Lovegrass
Autor/es:
ZAPPACOSTA, DIEGO CARLOS; GARBUS, INGRID; SELVA, JUAN-PABLO; CARBALLO, JOSÉ; PASTEN, MARÍA CIELO; GALLARDO, JIMENA; BELLIDO, ANDRÉS; MARCONI, G; ALBERTINI, EMIDIO; ECHENIQUE, VIVIANA
Lugar:
Bahía Blanca
Reunión:
Seminario; 7th Series of Seminars on Advances in Apomixis Research; 2020
Institución organizadora:
CERZOS
Resumen:
Weeping lovegrass (Eragrostis curvula) is a forage grass with a particular diplosporous apomixis where meiosis is absent and sexual and apomictic seeds have equal embryo:endosperm ploidy ratio. Several lines of evidence show that epigenetic regulation is involved in the expressiveness of the trait, accounting for the variable expression between plants and for the increase of the frequency of sexual pistils in facultative genotypes triggered by biotic and abiotic stresses. The aim of this study was to associate differences in the reproductive mode to epigenetic mechanisms given by DNA methylation and miRNAs regulation of gene expression. Firstly, the Methylation Content Sensitive Enzyme ddRAD (MCSeEd) was used to detect hypermethylated and hypomethylated positions and further associate them with apomictic (full and facultative) and sexual genotypes. The distribution of differentially methylated positions across genes at CG and 6mA shows a peak around the transcript start and transcript termination sites, hence regulating gene expression mainly through the incorporation of methyl groups on these positions. Gene ontology analysis reveals terms associated to auxins, clathrins, and kinases previously observed in apomictic species. Moreover, some genes differentially expressed related to the reproductive pathway, could be linked to the methylation status in the present work. Regarding miRNAs, a target analysis allowed the identification of a MADS-box transcription factor and a transposon repressed in the sexual genotype through specific miRNA-mRNA interactions. Additional miRNA-mRNA pairs specific for sexual or apomictic genotypes were identified in a degradome-based analysis conducted using Oryza sativa database, reinforcing the concept of apomixis resulting from sexual pathway deregulation