INVESTIGADORES
SELVA juan pablo
artículos
Título:
Novel genotypes of the subtropical grass Eragrostis curvula for the analysis of apomixis (diplospory)
Autor/es:
SUSANA CARDONE; PABLO POLCI; JUAN P. SELVA; MARTÍN A. MECCHIA; SILVINA PESSINO; PAULA HERMANN; VIVIANA CAMBI; PAUL W. VOIGT; GERMÁN SPANGENBERG; VIVIANA ECHENIQUE
Revista:
EUPHYTICA
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2006 vol. 151 p. 263 - 272
ISSN:
0014-2336
Resumen:
The aim of this work was to obtain a series of genetically related lines of Eragrostis curvula with different ploidy levels and reproductive modes, which can be used for the discovery of genes associated to diplospory. E. curvula is a widely cultivated forage grass native to Southern Africa that reproduces naturally by obligate diplosporous apomixis (asexual reproduction by seeds) of the Antennaria type. In vitro culture of immature inflorescences of the apomictic cultivar Tanganyika led to the regeneration of a diploid plant with a particular morphology (plant UNST1122). Embryo sac analysis and progeny tests determined that plant UNST1122 reproduces by sexuality. UNST1122 was cloned by dividing its tillers and a R1 generation was obtained from seeds after open pollination among the diploid clones. A set of 500 seeds from a diploid R1 plant was treated with colchicine and two plants showing a duplicated chromosome number (2n = 4x = 40) were obtained (plants UNST1112 and UNST1131). Progeny tests using RAPDs indicated that these plants reproduce also by sexuality. The availability of sexual tetraploid genotypes of E. curvula will allow the production of hybrids with new interesting combinations of agronomic traits and the developing of mapping populations segregating for diplospory. Besides, these genetically related plants with different reproduction modes constitutes an excellent model for the identification of gen(es) involved in diplosporous apomixis by mRNA profiling as well as to study the genomic rearrangements and gene expression alterations associated to changes at ploidy levels.