INVESTIGADORES
SARNACKI Sebastian Hernan
congresos y reuniones científicas
Título:
Salmonella infection induces changes in joints of mice inoculated by the oral route. The XII International Congress of Bacteriology and Applied Microbiology.
Autor/es:
NOTO LLANA, M; CACCURI, RL; GIACOMODONATO, MN; SARNACKI, SH; CERQUETTI, MC.
Lugar:
Estambul, Turquía.
Reunión:
Congreso; XII International Congress of Bacteriology and Applied Microbiology.; 2008
Institución organizadora:
International Union of Microbiological Societies (IUMS)
Resumen:
&amp;amp;lt;!-- /* Font Definitions */ @font-face {font-family:Tahoma; panose-1:2 11 6 4 3 5 4 4 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:1627421319 -2147483648 8 0 66047 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-US;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --&amp;amp;gt; Reactive arthritis (ReA) is a synovitis that overcomes generally after a gastrointestinal or urogenital tract infections. In some developing countries 33% of the ReA cases appeared after Salmonella intestinal infection. Currently, there is no suitable animal model for the study of ReA following gastrointestinal infections. In this work, we analyzed the capacity of Salmonella to induce joint lesions in BALB/c mice. Animals were intragastrically (ig) inoculated with 2 doses of 5x107 UFC of a termosensitive mutant C/2/2 of S. Enteritidis or one dose of 102- 104 UFC of the wild type strain. These sublethal doses produced histological changes compatible with enterocolitis. A group of animals was inoculated in the knee with zymosan before the infection in order to produce a lesion. At different times knee samples were taken. Bacteria were not recovered from the joints at any time studied. Histological assessment was performed using an established scoring system (0= normal joint, 1= simple hyperplasia of the synovial membrane, 2= synovitis with inflammatory infiltrate, 3= perisynovitis, 4= inflammatory infiltrate in joint cavity). Results showed that zymosan inoculation generates simple hyperplasia (index between 0 and 1) in days 14, 20, 43 and 75.Treatment with the attenuated strain exacerbated these histological changes, reaching an index between 2 and 3 (p<0.01 with respect to their controls). In joints without previous damage, the infection with the mutant produced minor changes. The joints of those animals inoculated only with the wt strain in a low dose showed a score between 2 and 3 at 25 and 42 days post challenge (p<0.01 compared to their controls). Specific antibodies against whole Salmonella were investigated in knee homogenates by Ouchterlony assay, although they were not detected. Our results show that the experimental infection with S. Enteritidis administered ig, generates ReA. High doses of attenuated strain of S. Enteritidis induce the reactivation of preexistent lesions, whereas low doses of the wt strain produce inflammatory changes in healthy joints.