Foodborne diseases by salmonella enterica serovar enteritidis during early stages of puerperium induce an adverse maternal outcome in the murine model

M NOTO LLANA; L KRUMMEL; A GARÓFALO; R MEISS; D BETANCOURT,; S SARNACKI; F BUZZOLA; M CERQUETTI; M GIACOMODONATO

CABA

Congreso; Reunión conjunta de Sociedades Biocientificas; 2017

Reunión conjunta de Sociedades Biocientificas

Diarrheal disease agents,especially non-typhoidal Salmonellaenterica (S. Enteritidis), are responsible forthe majority of deaths due to foodborne diseases (FBD). Puerperium is apredisposing condition for exacerbation of infections. Our aim was to study theeffect of FBD by S. Enteritidis during the earlypuerperium. To this purpose, we used an enterocolitis model. BALB/c micereceived 20 mg of streptomycin 48 hs postpartum. 24 hs later they received 3,500 CFU of S. Enteritidisintragastrically (sub lethal dose). At day 3 post infection (day 6 postpartum) intestinal permeability was measured in vivo. Gut samples were analyzed for bacterial colonization, histologicalchanges and tight junctions mRNA levels. Ileum and serum citoquines expressionwere studied by qPCR and ELISA, respectively. We found that 86% of puerperal females died within 5 days post infection whereas the mortality rate of infectedvirgin mice was 20%.This adverse maternal outcome is correlated with massivebacterial organs colonization. Also, gut permeability was significantlyincreased in infected parturient females (p<0.05) respect to control mice (non infected postpartum females, infected and non infected virgin females).Histology of serial cross sections showed irregular mucosa with decreasedvillus height, MALT hyperplasia and detriment of goblet cells versus nosignificant histological changes in non infected mice. Moreover, Claudin4 mRNA levelswere 3 fold higher in infected parturient mice than control groups (p<0.05) while Zona occludens showedno significant differences. IFN-γ and IL-17 expression was upregulated in infected maternal gut and serum vs control mice (p<0.05). Insummary, this work demonstrates that FBD by S. Enteritidis during earlystages of puerperium cause negative effects on host survival probably by the increasedgut permeability due to maternal immune response. Infected puerperal femalesare unable to self-limit the infection leading to fatal illness.