INVESTIGADORES
SARNACKI Sebastian Hernan
congresos y reuniones científicas
Título:
Prevention of enteritis and synovitis in Salmonella enterocolitis in mice fed with lactobacillus casei
Autor/es:
NOTO LLANA M; GARTNER AS; GIACOMODONATO MN; SARNACKI SH; AYA CASTAÑEDA MR; CERQUETTI MC
Lugar:
Portero de Funes, San Luis
Reunión:
Congreso; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular.; 2011
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB)
Resumen:
We investigated inflammatory responses
of intestine and knee joints in a mouse model for enterocolitis. Mice were
inoculated orally with 20 mg of streptomycin and 24 hs later they received 103 CFU of a virulent strain of Salmonella
Enteritidis by the
same route. Salmonella
was recovered from
Peyer´s patches (PP) up to day 4 and from spleen up to day 14. Bacteria were
neither recovered from knee joints nor from draining lymphnodes. Infected mice showed
a significant increase in intestinal IL-17 and TNF-a, determined by qPCR. 21 days
post-infection animals presented synovitis in the knee joints, suggesting that
this model of Salmonella enterocolitis is suitable for studying reactive
arthritis. We then tested the effect of probiotics in preventing the joint sequelae.
Mice were fed with commercially-available Lactobacillus casei fermented milk for 7 days before
infection. PP and spleen of mice pretreated with probiotics were less colonized
with Salmonella, and for a shorter period. Probiotic
feeding previous to Salmonella infection resulted in a significant decrease of gut
inflammatory cytokines, compared to infected animals not receiving Lactobacillus. Our results showed that the model for enterocolitis
might be useful for studying the pathogenesis of Salmonella-induced synovitis and that these
sequelae were prevented in mice pretreated with fermented milk containing L. casei.