INVESTIGADORES
SARNACKI Sebastian Hernan
artículos
Título:
Salmonella enterica induces joint inflammation and the expression of IL-17 in draining lymph nodes early after the onset of enterocolitis in mice
Autor/es:
MARIANGELES NOTO LLANA; SEBASTIAN H. SARNACKI; MARIA VICTORIA VAZQUEZ; ALEJANDRA GARTNER; MONICA N GIACOMODONATO; MARIA CRISTINA CERQUETTI
Revista:
INFECTION AND IMMUNITY
Editorial:
AMER SOC MICROBIOLOGY
Referencias:
Año: 2012 vol. 80 p. 2231 - 2239
ISSN:
0019-9567
Resumen:
In developing countries, one third of reactive arthritis (ReA) cases are associated with Salmonella enterocolitis, nevertheless there is no animal model for studying this pathology. Here we induced a self-limiting Salmonella enterica serovar Enteritidisenterocolitis in mice to analyze the onset of ReA. BALB/c mice received orally 20 μg of streptomycin 24 h before intragastric inoculation of a low dose (3-4 x 103 CFU) of S. Enteritidis. In response to Salmonella infection, a 30-fold increase in the expression of IL-17, measured by qPCR, was observed in mesenteric lymph nodes 5 days post infection. At this time synovitis was already evident and concomitantly a significant increase in joint TNF-α was detected by ELISA. The early development of joint lesions was accompanied by an increased expression of IL-17 in inguinal and popliteal lymph nodes. Infection with 107 CFU of an isogenic ΔinvG mutant bearing a defective TTSS-1 induced enterocolitis histologically similar to that triggered by the wild type strain. Interestingly, despite the higher infective dose used, the mutant did not trigger intestinal IL-17. Moreover, no synovitis was observed in mice suffering ΔinvG enterocolitis. Neutralization of IL-17 in mice infected with S. Enteritidis prevented both synovitis and the increment of TNF-α in the joints suggesting that IL-17 participates in the generation of Salmonella-induced ReA through the induction of TNF-α in the joints.