Cross-species amplification of seventeen polymorphic microsatellite loci in the endangered Crowned Eagle (Harpyhaliaetus coronatus)

SARASOLA, J.H; CANAL, D.; SOLARO, C.; GALMES, M.A.; ZANÓN MARTINEZ, J.I.; NEGRO, J.J.

MOLECULAR ECOLOGY RESOURCES

WILEY-BLACKWELL PUBLISHING, INC

Año: 2012 vol. 12 p. 779 - 781

1755-098X

The crowned eagle (Harpyhaliaetus coronatus) is one of the most severely threatened birds of prey in the world for which genetic markers have not been developed. We examined the cross amplification of thirty seven microsatellite loci in this endangered eagle. Seventeen loci were polymorphic and hence valuable as tools for population genetic studies. The number of alleles per locus ranged from 2 to 9, and the average number of alleles across all polymorphic loci was 4.4. The markers tested provide a valuable resource for research in population genetics and the conservation of this species. The success of cross-species amplification suggests that these microsatellites will be useful for studies in a broad range of raptor species. Harpyhaliaetus coronatus) is one of the most severely threatened birds of prey in the world for which genetic markers have not been developed. We examined the cross amplification of thirty seven microsatellite loci in this endangered eagle. Seventeen loci were polymorphic and hence valuable as tools for population genetic studies. The number of alleles per locus ranged from 2 to 9, and the average number of alleles across all polymorphic loci was 4.4. The markers tested provide a valuable resource for research in population genetics and the conservation of this species. The success of cross-species amplification suggests that these microsatellites will be useful for studies in a broad range of raptor species.