Moving forward Strongyloides stercoralis detection, studying molecular typing as infection follow up strategy in immunocom promised patients

REPETTO SILVIA ANALÍA; ARGÜELLO LISANA BELÉN; BATALLA ESTELA; BURGOS JUAN MIGUEL; GONZALEZ CAPPA STELLA MARIS; ALBA SOTO CATALINA DIRNEY; RISSO MARIKENA GUADALUPE; RUYBAL PAULA

Congreso; 18th ICID | International Congress on Infectious Diseases; 2018

Background: Strongyloides stercoralis is a geohelminth which affects 10-40% of the world population in tropical and subtropical areas. It produces chronic infections and severe symptomsin immunocompromised patients with high mortality. Although the parasitological cure is defined as the absence of larvae after one year of treatment, we have observedreactivations after the second year. We evaluated genetic diversity and its possible association with the clinical characteristics and evolution of this parasitosis.Methods & Materials: Twenty-two patients (18 immunocompromised) with diagnosis and follow-up of strongyloidosis from Argentina, Bolivia, Paraguay, Peru and Dominican Republic wereevaluated. The DNA extracted from stool sample at the time of diagnosis was used as a template for amplification and sequencing of a 404 bp region of the mitochondrialgene cox1. The analysis of sequences was: consensus assembly (STADEN), alignment (MEGA6), haplotype resolution (PHASE, DNAsp), allele coding and discriminatorypower calculation (PD, MLSTest). Sequences were analyzed in the context of sequences of S. stercoralis (581), S. fuelleborni, S. ratti, S. venezuelensis, S. planiceps, S.mirzai and S. papillosus. Results were expressed in frequencies and percentages. Level of significance was p0.05). However, in allimmunocompromised patients who reactivated (7/18), the HP24 variant of the parasite was detected. The presence of HP24 increased the risk of reactivation with a RR of2.16 (p