INVESTIGADORES
RUYBAL paula
congresos y reuniones científicas
Título:
Attenuated Salmonella entérica serovar Typhi (S. Typhi) invades and induces cell death in a T-cell lymphoma
Autor/es:
GRAVISACO MARÍA JOSÉ; RUYBAL PAULA; CROCI MÁXIMO; PASETTI MARCELA; MONGINI CLAUDIA; WALDNER CLAUDIA
Lugar:
Tandil, Buenos Aires, Argentina
Reunión:
Congreso; XXXIV Reunión anual de la Sociedad Argentina de Farmacología Experimental; 2002
Institución organizadora:
Sociedad Argentina de Farmacología Experimental
Resumen:
Salmonella is a facultative intracellular pathogen that offers several advantages to be considered a potential anticancer vaccine. They can grow under either aerobic or anaerobic conditions, such as those that occur within solid tumors; they express specialized systems for invasion and survival within both epithelial cells and macrophages. The uptake of Salmonella into non-professional phagocytes is an active process induced by the bacteria. In this work we investigated the ability of attenuated S. Typhi to invade T-lymphoma cells in order to use it as an antitumor vector. Tumor cells were implanted subcutaneously into naive mice and ten days later 2 x 109 CFU of attenuated S. Thypi were inoculated intratumor. Animals were euthanized at different days and the tumors were removed. Several portions of the tumor were prepared for microscopic evaluation. Apoptosis assays were performed. The remainder of the tumor was homogenized and lysed to quantify intracellular bacteria. Our results showed that attenuated S. Typhi can invade and survive within tumor cells in vivo. Furthermore, this bacterial strain was able to induce tumor cell death by mechanisms of apoptosis and necrosis. These findings suggest that this attenuated Salmonella strain could be useful to induce inherent antitumor activity as well as to deliver in vivo therapeutic DNA or proteins to cancer cells.