INVESTIGADORES
RUYBAL paula
artículos
Título:
Characterization of the immunophenotype and the metastatic properties of a murine T-lymphoma cell line
Autor/es:
MONGINI CLAUDIA; RUYBAL PAULA; GRAVISACO MARIA JOSE; CROCI MAXIMO; SÁNCHEZ LOCKHART MARIANO; FABRIS VICTORIA; WALDNER CLAUDIA
Revista:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. ANIMAL.
Editorial:
SPRINGER
Referencias:
Año: 2001 vol. 37 p. 499 - 504
ISSN:
1071-2690
Resumen:
We report the first characterization of a mouse T-lymphoma cell line that surprisingly expresses  cytoplasmatic (cy) cyCD4. Phenotypically, LBC cells are CD51, CD81, CD161, CD241, CD251, CD22/dim, CD32/dim, TCRb2/dim, TCRgd2, CD1542, CD402, and CD45R2. Coexpress cyTCRb, cyCD3, cyCD4, and yet lack surface CD4 expression. Transplantation of LBC cells into mice resulted in an aggressive T-lymphoblastic lymphoma that infiltrated lymph nodes, thymus, spleen, liver, ovary, and uterus but not peripheral blood or bone marrow. LBC cells display a modal chromosome number of 39 and a near-diploid karyotype. Based on the characterization data, we demonstrated that the LBC cell line was derived from an early T-cell lymphocyte precursor. We propose that the malignant cell transformation of LBC cells could coincide with the transition stage from late double-negative, DN3 (CD42, CD82CD442/low, CD251) or DN4 (CD42/low, CD82/low, CD442, CD252) to double-positive (DP: CD41CD81) stage of T-cell development. LBC cells provide a T-lymphoblastic lymphoma model derived from a malignant early T-lymphocyte that can be potentially useful as a model to study both cellular regulation and differentiation of T-cells. In addition, LBC tumor provides a short latency neoplasm to study cellular regulation and to perform preclinical trials of lymphoma-related disorders. 1, CD81, CD161, CD241, CD251, CD22/dim, CD32/dim, TCRb2/dim, TCRgd2, CD1542, CD402, and CD45R2. Coexpress cyTCRb, cyCD3, cyCD4, and yet lack surface CD4 expression. Transplantation of LBC cells into mice resulted in an aggressive T-lymphoblastic lymphoma that infiltrated lymph nodes, thymus, spleen, liver, ovary, and uterus but not peripheral blood or bone marrow. LBC cells display a modal chromosome number of 39 and a near-diploid karyotype. Based on the characterization data, we demonstrated that the LBC cell line was derived from an early T-cell lymphocyte precursor. We propose that the malignant cell transformation of LBC cells could coincide with the transition stage from late double-negative, DN3 (CD42, CD82CD442/low, CD251) or DN4 (CD42/low, CD82/low, CD442, CD252) to double-positive (DP: CD41CD81) stage of T-cell development. LBC cells provide a T-lymphoblastic lymphoma model derived from a malignant early T-lymphocyte that can be potentially useful as a model to study both cellular regulation and differentiation of T-cells. In addition, LBC tumor provides a short latency neoplasm to study cellular regulation and to perform preclinical trials of lymphoma-related disorders.