INVESTIGADORES
RUYBAL paula
artículos
Título:
Leptospira species molecular epidemiology in the genomic era
Autor/es:
CAIMI KARINA; REPETTO SILVIA ANALÍA; VARNI VANINA; RUYBAL PAULA
Revista:
Infection, Genetics and Evolution
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2017 vol. 54 p. 478 - 485
ISSN:
1567-1348
Resumen:
Leptospirosis is a zoonotic disease with a global burden that has increased due to growing poverty and heavily seasonal rainfalls. To date, the whole genome sequences of hundreds of Leptospira spp. isolates collected worldwide are available, together with online tools that permit to assign MLST sequence types (STs) directly from raw sequence data. In this work, we have applied R7L-MLST to 527 genomes and strain collections globally distributed. All the pathogenic (N=10) and intermediate species were typed using this MLST scheme. The correlation observed between STs and serogroups in our previous work is still satisfied with this higher dataset, thus sustaining the implementation of MLST to assist serological classification as a complementary approach. Bayesian phylogenetic analysis of concatenated sequences from R7-MLST loci allowed us to resolve taxonomic inconsistencies, and also showed that events such as recombination, gene conversion or lateral gene transfer played an important role in the evolution of the Leptospira genus. This study could contribute to suitable epidemiologic information for the design of control strategies and in diagnostic methods for this illness.