Molecular typing of Argentinian Mycobacterium avium subsp. paratuberculosis isolates by multiple-locus variable number-tandem repeat analysis

GIOFFRE ANDREA; CORREA MUÑOZ MAGNOLIA; ALVARADO PINEDO MARIA FIORELLA; VACA ROBERTO; MORSELLA CLAUDIA; FIORENTINO MARÍA ANDREA ; PAOLICCHI FERNANDO; RUYBAL PAULA; ZUMARRAGA MARTIN; TRAVERÍA GABRIEL EDUARDO; ROMANO MARIA ISABEL

BRAZILIAN JOURNAL OF MICROBIOLOGY

SOC BRASILEIRA MICROBIOLOGIA

Año: 2015 vol. 46 p. 557 - 564

1517-8382

Multiple-locus variable number-tandem repeat analysis (MLVA) of Mycobacterium avium subspecies paratuberculosis (MAP) isolates may contribute to the knowledge of strain diversity in Argentina. Although the diversity of MAP has been previously investigated in Argentina usingIS900-RFLP, a small number of isolates were employed, and a low discriminative power wasreached. The aim of the present study was to test the genetic diversity among MAP isolates using anMLVA approach based on 8 repetitive loci. We studied 97 isolates from cattle, goat and sheep andcould describe 7 different patterns: INMV1, INMV2, INMV11, INMV13, INMV16, INMV33 andone incomplete pattern. INMV1 and INMV2 were the most frequent patterns, grouping 76.3% of theisolates. We were also able to demonstrate the coexistence of genotypes in herds and co-infection atthe organism level. This study shows that all the patterns described are common to those described inEurope, suggesting an epidemiological link between the continents.