ROLE OF 90KDA HEAT SHOCK PROTEIN IN PLANT IMMUNITY

CORIGLIANO, M; ROSSI FR; CISTARI, NS; SANCHEZ LOPEZ, EF; GARRIZ A; CLEMENTE, M

Congreso; Argentine Society for Biochemistry and Molecular Biology; 2016

Several works demonstrated that plant Hsp90s have an activerole in the activation of the plant defense mechanisms againstpathogens, since the lack or deficit of these proteins impairsdefense against pathogenic bacteria and fungus. Hsp90s arefound in all plant cellular compartments examined to date, including cytosol, nucleus, endoplasmic reticulum, mitochondriaand chloroplasts. Recently, Hsp90 have also been found in theapoplast, even though their role in this location has not beendescribed. In this work, we added some light to this issue byassessing the effects on the defense mechanisms occurring in Arabidopsis thaliana plants infiltrated with a recombinantHsp81.2 protein from A. thaliana (rAtHsp81.2). After 24 h, cell death, ROS and callose were accumulated in infiltrated leaves, suggesting that the accumulation of the protein may participatein the induction of defense. In this sense, a 24h pre-treatmentofleaves with rAtHsp81.2 resulted in a reduction in theproliferation of Pseudomonas syringaepv. tomato DC3000 (PstDC3000), whereas the in vitro growth of the bacteria is notaffected in the presence of rAtHsp81.2. Our results suggest that infiltration with recombinant Hsp90 is important for mountingappropriate plant immune responses