Cellulase production by solid state fermentation and its separation with polyacrylate
GARCIA GALINDO, A. IVANOE; MEDINA MORALES, MIGUEL; ROMANINI, DIANA; PICÓ, GUILLREMO; AGUILAR, CRISTOBAL
ELSEVIER SCIENCE BV
Lugar: Amsterdam; Año: 2016 vol. 33 p. 429 - 429
Aspergillus niger GH1 is reported in the literature as an important producer of extracellular enzymes in Solid State Fermentation (SSF) in many organic substrates with good yields. Mexico in 2014, 25,500,000 tons of corn waste were produced. For that reason, in this work the corn cob was considered as an excellent support for SSF. Through this fermentation system, a multi-enzymatic extract was produced, with high activity after 96 hours of fermentation. One of the enzyme activities was expressed as cellulolytic activity. For the recovery of the enzymes, the process performed in one step of precipitation of enzymes with a negatively charged (polyacrylate PAA 240,000) polymer, applied directly to an extract of A. niger GH1. This allowed an acceptable recovery of the enzymes and a high purification factor in the precipitate with endo and exoglucanase activities. The optimum conditions of precipitation of the enzymes with PAA were as follows: 400 ml of PAA (0.05%, w/w) pH 3.00, 2 ml of phosphate buffer, pH 3.00, and 2.4 ml of enzyme extract pH 3.00. The highest results of recovery performance were obtained for exoglucanase activity.