INVESTIGADORES
SOMOZA Gustavo Manuel
congresos y reuniones científicas
Título:
Expression of kisspeptin 2 and receptor 2b during early larval stages in pejerrey (Odontesthes bonariensis).
Autor/es:
OSWALDO TOVAR; ALEJANDRO S. MECHALY; LUIS F. CANOSA; GUSTAVO M. SOMOZA
Lugar:
Chascomus
Reunión:
Congreso; XVI Jornadas Anuales de la Sociedad Argentina de Biología; 2014
Institución organizadora:
Sociedad Argentina de Biología
Resumen:
Kisspeptin is considered a gatekeeper in the regulation of gonadal maturation and puberty onset in mammals. However its role on fish reproduction is not so clear and there are few studies about the role of this system during early developmental stages. Previous studies revealed the presence of two different isoforms of Kiss2 and Kissr2 in some teleost species. In this context the objective of this work was to search for Kiss2 and Kissr2 isoforms and measure their expression levels during the first weeks after hatch (wah) in pejerrey. We could only detect splicing variants in Kissr2b but we designed specific primers for one exon (P1) and in an exon-exon junction (P2) for both genes (Kiss2 and Kissr2). Pejerrey fry were maintained at 17ºC, 24ºC and 29ºC during 8 wah. Five individuals were collected weekly and cDNA were obtained from heads, and then RT-QPCR was performed and normalized quantities were calculated. We found Kiss2 and Kissr2b expression since week 1 (W1) to week 8 (W8). When the samples were analyzed with P1 primers we found significant increases at 17ºC for Kiss2 at W2 and for Kiss2rb at W3. However, when the same samples were analyzed with P2 primers, no differences were detected. In conclusion we report a early expression of spliced variants of Kiss2 and Kissr2b mRNA in pejerrey. The correct design of the primers is important to measure biologically active isoforms.