INVESTIGADORES
SOMOZA Gustavo Manuel
congresos y reuniones científicas
Título:
Serotonin modulates proliferation of radial glial cells in the hypothalamus of adult zebrafish (Danio rerio).
Autor/es:
MARÍA R. PÉREZ; ELIZABETH PELLEGRINI; MARIE MADELAINE GUEGUEN; COLETTE VAILLANT; GUSTAVO M. SOMOZA; OLIVIER KAH
Lugar:
Buenos Aires
Reunión:
Congreso; 7th International Symposium on Fish Endocrinology.; 2012
Resumen:
Serotonin (5-HT) is a major neurotransmitter in the central nervous systems.In teleost fish, 5-HT neurons have a wide distribution and are abundant in brainregions exhibiting proliferative activity, such as the hypothalamic nucleus of theposterior recess (NRP). In order to understand the functional link between 5-HTand neurogenesis in fish, we combined neuroanatomical and BrdU experiments inadult zebrafish. We focused our investigation in the NRP which contains radial glialcells (RGC) that actively divide. Using a transgenic zebrafish line expressing GFP inRGC, we showed that 5-HT neurons were positioned between the ventricle and theRGC and were of the cerebrospinal fluid-contacting type. In vivo BrdU incorporationexperiments have demonstrated that 5-HT neurons could be generated from RGC.5-htr1ab mRNA were visualized in the NRP close to/or in RGC. To investigate whetherthe 5-HT modulates the proliferation, p-chlorophenylalanine (pCPA) treatmentsdesigned to reduce endogenous 5-HT were performed. The number of proliferatingcells significantly decreased in the NRP after pCPA administration. These resultssuggest a close functional relationship between 5-HT neurons, RGC and proliferativeactivity in the zebrafish brain.