Molecular characterization of Kisspeptins in Odontesthes bonariensis.

OSWALDO TOVAR; LUIS F. CANOSA; GUSTAVO M. SOMOZA

Buenos Aires

Congreso; 7th International Symposium on Fish Endocrinology.; 2012

During the last seven years the Kisspeptin system has been studied mainly in fishand mammals due to its importance in reproduction and puberty. The objective ofthis study was to characterize this system in an atherinopsidae fish, O. bonariensis,commonly named as pejerrey. Adult pejerrey from the IIB-INTECH broodstockwere sacrificed, the brain and pituitary gland removed and stored separately at-80°C until RNA extraction. We have first obtained short fragments of kisspeptin1(kiss1), kisspeptin2 (kiss2) and kisspeptin receptor (kissR) using consensus anddegenerated primers. The obtained fragments were 127 pb for kiss1, 275 pb forkiss2 and 873 pb for kissR. Then, the 3´ and 5´ends were amplified by the RapidAmplification cDNA Ends (RACE) method using specific primers for kiss1, kiss2 andkissR. In addition, specific primers were used for studying kisspeptins and kissRtissue distribution. The phylogenetic analyses of kiss1, kiss2 and kissR will bepresented. Pejerrey kisspeptins and kissR partial sequences showed high homologyto kisspeptins mRNA from Perciform species.