INVESTIGADORES
SOMOZA Gustavo Manuel
artículos
Título:
A cheap and quick method for DNA-based sexing of birds.
Autor/es:
FLAVIO QUINTANA; GABRIELA LÓPEZ; GUSTAVO M. SOMOZA
Revista:
WATERBIRDS (DE LEON SPRINGS, FLA.)
Editorial:
Waterbird Society
Referencias:
Año: 2008 vol. 31 p. 485 - 488
ISSN:
1524-4695
Resumen:
Although the collection of air-dried blood samples on filter paper has been used for field work as a routine, it has required the use of special and expensive types of paper. In the present manuscript the use of common filter paper to collect and store air-dried bird blood samples is validated for its use in DNA based sexing techniques with a very simple method avoiding DNA purification for PCR. This method not only does simplify the laboratory work but also does not add any additional cost for DNA based sexing techniques. The method was tested and successfully employed for sex determination by molecular techniques on blood samples taken on common filter paper during different field trips from 4 seabird species.