Effects on growth, development and abnormalities induced by a glyphosate commercial formulation and its active ingredient on two developmental stages of the South-American common frog, Leptodactylus latrans.

NADIA C. BACH; GUILLERMO S. NATALE; GUSTAVO M. SOMOZA; ALICIA RONCO

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH

SPRINGER HEIDELBERG

Lugar: HEIDELBERG; Año: 2016 vol. 23 p. 23959 - 23971

0944-1344

In this study we evaluated acute lethal and sublethal effects of glyphosate technical grade (GLY) and the glyphosate-based commercial formulation Roundup® Ultramax (RU) on two Gosner-stages ([Gs] 25 and 36) of the South-American common frog, Leptodactylus latrans. Bioassays were performed following standardized methods within a large range of concentrations (0.00074 to 9.62 mg of acid equivalents per litre -a.e./L- of RU; and between 3 and 300 mg/L of GLY). The evaluated endpoints were mortality, swimming activity, growth, development and presence of morphological abnormalities, with special emphasis on oral abnormalities. No lethal effects were observed on larvae exposed to GLY neither in Gs 25 nor in 36. The LC-50 values in RU exposed larvae at different exposure times and Gs ranged from 3.26 to 9.61 mg a.e./L. Swimming activity was only affected by RU. Effects on growth, development and presence of morphological abnormalities ?like oral abnormalities and oedemas- were observed under both GLY and RU exposures. Gs 25 was the most sensitive stage to both forms of the herbicide. Commercial formulation of the herbicide was much more toxic than the active ingredient on all assessed endpoints. Effects on growth, development and presence of morphological abnormalities observed in the range of environmental concentrations reported for agroecosystems of Argentina, alert on the potential effects of the herbicide that could be affecting the fitness and survival of anurans in agroecosystems.