Presence of 11-ketotestosterone in pre-differentiated male gonads of Odontesthes bonariensis

MARTÍN BLASCO; GUSTAVO M. SOMOZA; DENISE VIZZIANO-CANTONNET

FISH PHYSIOLOGY AND BIOCHEMISTRY

SPRINGER

Lugar: Berlin; Año: 2013 vol. 39 p. 71 - 74

0920-1742

The involvement of androgens during sex differentiation period was investigated in thepejerrey Odontesthes bonariensis, by classic biochemical studies and gonad histology. Westudied in particular whether the enzymes activities involved in 11-oxygenated androgenproduction were active in a gonadal/peritoneum complex (GPC) of very small larvaeexposed to masculinizing temperatures previous to morphological sex differentiation (5weeks post-hatching). The GPC was incubated with 17-hydroxyprogesterone (3H-17P) andthe presence of 11-KT as major metabolite in early gonads undergoing masculine pathwayafter temperature treatment exposure is reported. 11-KT was identified by TLC and HPLC.The present results shows that 11KT is produced at very early stages of testis developmentin pejerrey, being this androgen one of the main mediators of the masculinization inducedby temperature treatment at the gonad level.