INVESTIGADORES
SOMOZA Gustavo Manuel
artículos
Título:
A Y-linked anti-Müllerian hormone duplication takes over a critical role in sex determination
Autor/es:
RICARDO S. HATTORI; YU MURAI; MIHO OURA; SHUJI MASUDA; SULLIP MAJHI; TAKASHI SAKAMOTO; JUAN I. FERNANDINO; GUSTAVO M. SOMOZA; MASASHI YOKOTA; CARLOS A. STRÜSSMANN
Revista:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Editorial:
NATL ACAD SCIENCES
Referencias:
Año: 2012 vol. 109 p. 2955 - 2959
ISSN:
0027-8424
Resumen:
Gonadal sex determination in vertebrates generally follows a sequence of  genetically programmed events. In what is seemingly becoming a pattern, all confirmed or current candidate “master” sex determining genes reported in this group, e. g. SRY in eutherian mammals, DMY/dmrt1bY inmedaka, DM-W in African clawed frog, and DMRT1 in chicken encode transcription factors. In contrast, here we show that a male-specific, duplicated copy of the anti-Müllerian hormone (amh) is implicated in testicular development oif the teleost fish Patagonian pejerrey Odontesthes hatcheri. The gene, termed amhy for it is found in a single metacentric/submetacentric chromosome of XY individuals, is expressed much earlier than the autosomal amh (6 days after fertilization vs 12 weeks after fertilization) and is localized to presumptive Sertoli cells of XY males during testicular differentiation. Moreover, amhy knockdown in XY embryos resulted in the upregulation of foxl2 and cyp19a1a mRNAs and the development of ovaries. These results are the first evidence of afunctional amh duplication in vertebrates and suggest that amhy may be the master sex-determining gene in this species. If confirmed, this would be the first report ever of a hormone-related gene, a member of the TGF-β superfamily, in such a role.