INVESTIGADORES
SOMOZA Gustavo Manuel
artículos
Título:
Rapid separation of gonadotropin-releasing hormone molecular forms by isocratic high-performance liquid chromatography on an ion-exchange column.
Autor/es:
JOSE L D ERAMO; GUSTAVO M SOMOZA; ANDREA STEFANO; LUIS F CANOSA; OSVALDO FRIDMAN; GUSTAVO M SOMOZA
Revista:
JOURNAL OF CHROMATOGRAPHY B
Editorial:
ELSEVIER
Referencias:
Año: 1996 vol. 682 p. 219 - 224
ISSN:
0378-4347
Resumen:
The purpose of the present work was to develop a chromatographic system for the separation of five molecular forms of the gonadotropin-releasing hormone (GnRH); mammalian GnRH (mGnRH) (LHRH), salmon GnRH (sGnRH), chicken I GnRH (cIGnRH), chicken II GnRH (cIIGnRH) and lamprey GnRH I (lGnRH-I). By using an ion-exchange HPLC column and isocratic elution, it was possible to separate properly the five peptides in approximately 20 min. The utility of the systemin determining the GnRHs forms present in the brain of two species of vertebrates was examined.