Chlorophyll Fluorescence and Lipid Peroxidation Changes in Rice Somaclonal lines Subjected to Salt Stress

MONICA RACHOSKI; AYELÉN GÁZQUEZ; PABLO CALZADILLA; RODOLFO BEZUS; ANDRES ALBERTO RODRIGUEZ; OSCAR ADOLFO RUIZ; ANA BERNARDINA MENÉNDEZ; MAIALE SANTIAGO

ACTA PHYSIOLOGIAE PLANTARUM

SPRINGER HEIDELBERG

Año: 2015

0137-5881

The aim of the present work was to explore physiological changes provoked bysomaclonal variation in response to salinity. Two parental cultivars (La Candelaria andYerua) and their derived somaclones were used as a source for breeding new ricelines with improved salt tolerance. We studied the effect of NaCl salt stress onchlorophyll fluorescence-related parameters, such as the maximum quantum yield ofprimary PSII photochemistry (Fv/Fm) and the performance index for energyconservation from photon absorbed by PSII antenna (PIABS). In additionmalondialdehyde (MDA) content and leaf temperature (LT) responses were alsomeasured. In somaclonal lines, Fv/Fm, PIABS, MDA and LT showed coefficients ofvariation of 13,7%, 39,3%, 25,5%, and 3 % respectively for La Candelaria and 1,4%,17,6%, 34,4% and 3% for Yerua. However, the fragrant character did not differ in thearomatic somaclonal lines with respect to their parentals. Our results suggest that theFv/Fm ratio would not be as good marker of PSII vitality as PIABS for salinized ricesomaclones, unless they be highly susceptible to salinity. On other hand, the MDAcontent showed a strong negative correlation with the PIABS content in somaclones ofboth rice cultivars, suggesting that MDA levels could also be used as an oxidativedamage index in rice somaclones.