Regulation of biofilm aging and dispersal in Bacillus subtilis by the alternative sigma factor SigB

BARTOLINI M.; COGLIATI S.; VILETA D.; BAUMAN C.; RATENI L.; LEÑINI C.; GRAU R.

JOURNAL OF BACTERIOLOGY

AMER SOC MICROBIOLOGY

Lugar: Washington; Año: 2018

0021-9193

Bacterial biofilms are important in natural settings, biotechnology and medicine. However, regulation of biofilm development and its persistence in different niches is complex and only partially understood. One key step during the biofilm life cycle is dispersal, when motile cells abandon the mature biofilm to spread out and colonize new niches. Here, we show that in the model bacterium Bacillus subtilis, the general stress transcription factor SigB is essential for halting detrimental overgrowth of mature biofilm and for triggering dispersal when nutrients become limited. Specifically, SigB-deficient biofilms were larger than wild-type biofilms but exhibited accelerated cell death, significantly greater sensitivity to different stresses and reduced dispersal. Interestingly, the nutritional signal for biofilm fate detected by SigB was transduced through the RsbP-dependent metabolic arm of the SigB regulatory cascade, which in turn positively controlled expression of SinR, the master regulator of biofilm formation and cell motility. This novel SigB-SinR regulatory circuit might be important in controlling the fitness of biofilms (either beneficial or harmful) in diverse environments.