GRAU roberto Ricardo
Disruption of the gene spo0A encoding sporulation transcription factor blocks endospore formation and enterotoxin production....
HUANG I., WATERS M., GRAU R., AND SARKER M.
FEMS MICROBIOLOGY LETTERS
Blackwell - London
Año: 2004 vol. 233 p. 233 - 240
This study identified a functional spo0A ORF in enterotoxigenic Clostridium perfringens type A. To evaluate the function of spo0A, an isogenic knock-out mutant was constructed. The spo0A mutant was unable to form endospores and produce enterotoxin, however, these defects could be restored by complementing the mutant with a recombinant plasmid carrying the wild type spo0A gene. These results provide evidence that spo0A expression is essential for sporulation and enterotoxin production in C. perfringens.