Proteomic analysis of Amycolaptosis tucumanensis in response to chromium and copper stress

L. B. VILLEGAS; DELFINI C. D,; SCHÜTZE E,; KOTHE E; AMOROSO M.J ; ABATE C.M

Puerto Madryn

Congreso; XLVI Reunión Anual-Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010

Sociedad Argentina de Investigacion en Bioquímica y Biología Molecular

Heavy metal pollution is one the most serious environmental problems. Some heavy metals play an important biological role as trace elements but are toxic at higher concentrations. Amycolatopsis tucumanensiswas isolated from contaminated sediments in Argentina. Its ability to remove Cu(II) and Cr(VI) has been reported. The aim of this work was to analize the differential protein espression of A. tucumanensis under Cu(II) or/and Cr(VI) stress by two-dimensional polyacrylamide gel elctrophoresis. Cells were incubated in minimal medium containing glucose and 10 ppm of Cu(II) or/and Cr(VI) during 72 h at 30 ºC and 200 rpm. Cells grown in absence of heavy metals were used as control. The cells were chilled in liquid N2 and broken by physical technique. The supernatants of the cell lysates was used as protein sample. The proteome profiles were different, 18 spots were selected for the identification by mass spectrometry, and these included: dehydrogenase/reductase, alkyldroperoxidase Aph D, probable monoxigenase, RNA polimerase, cicloisomerase, some elongation factors, transcripcional regulator and several proteins with unknown function. Cu(II) or Cr(VI) resistance mechanisms are not known clearly, for this reason is very important to study the functional analysis of these identified proteins and will allow to explain the tolerance of heavy metals by this bacterium.