DISEÑO DE CEBADORES DEGENERADOS PARA EL FACTOR DE TRANSCRIPCION ACE-1 Y DETERMINACION DE ISOENZIMAS INDUCIBLES CON Cu+2 EN Peniophora sp.

FONSECA MI; RAMOS AB; TEJERINA M; FARIÑA JI; VILLALBA LL; ZAPATA PD

Viña del Mar

Congreso; XIV CONGRESO LATINOAMERICANO DE GENÉTICA (ALAG); 2010

SOCIEDAD DE GENÉTICA DE CHILE (SOCHIGEN), SOCIEDAD ARGENTINA DE GENÉTICA (SAG), ASOCIACIÓN LATINOAMERICANA DE MUTAGÉNESIS, CARCINOGÉNESIS Y TERATOGÉNESIS AMBIENTAL (ALAMCTA)

Abstract: The industry of pulp and paper is characterized by its constant searching for alternative processes which were compatible with the environment, effective and inexpensive. The White rot fungi are excellent ligninolytic organisms due to its enzyme secretion, which includes the Laccase (Lac). Researchers are currently being focused mainly towards the study of the regulatory elements of transcription and induction of Lac. Several cupper-binding sites in Lac have been discovered and the presence of DNA sites coding a transcription factor in response to Cu+2 (ACE-1) in Phanerochaete chrysosporium (Genbank accession number ABF60559.1) and in others Basidiomycetes has been demonstrated, which could activate the laccase gene transcription. Peniophora sp. BAFC 633 secretes Lac induced by Cu+2 which could be regulated by the ACE-1. The objective of this study was to design degenerate primers for amplifying the ace-1 in Peniophora sp and to determine the isoenzymatic profile on cultures induced with Cu+2.: The industry of pulp and paper is characterized by its constant searching for alternative processes which were compatible with the environment, effective and inexpensive. The White rot fungi are excellent ligninolytic organisms due to its enzyme secretion, which includes the Laccase (Lac). Researchers are currently being focused mainly towards the study of the regulatory elements of transcription and induction of Lac. Several cupper-binding sites in Lac have been discovered and the presence of DNA sites coding a transcription factor in response to Cu+2 (ACE-1) in Phanerochaete chrysosporium (Genbank accession number ABF60559.1) and in others Basidiomycetes has been demonstrated, which could activate the laccase gene transcription. Peniophora sp. BAFC 633 secretes Lac induced by Cu+2 which could be regulated by the ACE-1. The objective of this study was to design degenerate primers for amplifying the ace-1 in Peniophora sp and to determine the isoenzymatic profile on cultures induced with Cu+2.